An efficient fermentation process was developed for GlcNAc production using an E. coli strain containing integrated T7lac-glmS*54 and T7lac-GNA1 expression cassettes. Fermentation in one-liter fermentors was run in a fed batch mode. Cells were grown to an OD600 of about 28 and induced with lactose. GlcNAc levels in the broth reached 110 g L-1 at 72 hours.