Table of Contents
Abstract, page 1
Figure 1. Metabolic Engineering for N-Acetylglucosamine Production in E. coli, page 2
Table 1. Expression of Different Recombinant Glucosamine Synthase Genes in E. coli, page 3
Figure 2. GlcN Production by Over-Expressing GlmS Enzymes Resistant to Product Inhibition, page 4
Table 2. GlcNAc Production by Over-Expressing Glucosamine Synthase and Glucosamine-N-Acetyltransferase (GNA1), page 5
Figure 3. Lactose-Induced GlcNAc Production, page 6
Figure 4. Construction of nagB Over-Expression E. coli Strains, page 7
Figure 5. Suppression of GlcN Auxotrophy in glmS Mutants by nagB Over-Expression, page 8
Table 3. Production of GlcNAc by Over-Expressing both nagB and GNA1 Genes, page 9
Table 4. In Vitro Synthesis of GlcN and GlcNAc, page 10
Conclusions, page 11
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