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From Concept to Process: Metabolic Engineering for Production of Glucosamine and N-Acetylglucosamine

Author: Ming-de Deng, Ph.D. (info@biotechresources.com)
Presented at the Society for Industrial Microbiology Annual Meeting; July 28, 2004; San Diego, California USA
©2004 Bio-Technical Resources, all rights reserved.

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Table of Contents

Abstract, page 1

Introduction, page 2

Figure 1. Metabolic Engineering Strategy to Produce Glucosamine, page 3

glmS Gene Over-Expression in a Modified E. coli Host, page 4

Directed Evolution of GlmS Enzyme to Reduce Product Inhibition, page 5

Glucosamine Fermentation Process, page 6

Challenges of the Glucosamine Process, page 7

Concept of N-Acetylglucosamine Fermentation, page 8

N-Acetylglucosamine Fermentation Process, page 9

NAG Hydrolysis to Glucosamine, page 10

Conclusions, page 11

 

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