Author: Ming-de Deng, Ph.D. (mddeng@biotechresources.com)
Presented at the Society for Industrial Microbiology Annual
Meeting; July 28, 2004; San Diego, California USA
©2004 Bio-Technical Resources, all rights reserved.
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Table of ContentsAbstract, page 1 Introduction, page 2 Figure 1. Metabolic Engineering Strategy to Produce Glucosamine, page 3 glmS Gene Over-Expression in a Modified E. coli Host, page 4 Directed Evolution of GlmS Enzyme to Reduce Product Inhibition, page 5 Glucosamine Fermentation Process, page 6 Challenges of the Glucosamine Process, page 7 Concept of N-Acetylglucosamine Fermentation, page 8 N-Acetylglucosamine Fermentation Process, page 9 NAG Hydrolysis to Glucosamine, page 10 Conclusions, page 11 |
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