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Production of Farnesol and Geranylgeraniol by Strains of Saccharomyces cerevisiae

Author: Thomas W. McMullin, Ph.D. (info@biotechresources.com)
Presented at the Society for Industrial Microbiology Annual Meeting; July 30, 2001; St. Louis, Missouri USA
©2004 Bio-Technical Resources, all rights reserved.

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Table of Contents

Abstract, page 1

Introduction, page 2

Generation of Squalene Synthase Mutants Using Classical Methods, page 3

Wild Type and erg9 Mutant, page 4

MBNA1-13 Fed Batch Fermentation, page 5

Isoprenoid Pathway in Saccharomyces cerevisiae, page 6

Strain Genotypes, page 7

Amplification of GGPP and FPP Synthases, page 8

Effect of Amplifying Deregulated HMG CoA Reductase, page 9

Amplification of Isoprenoid Pathway, page 10

Conclusions, page 11



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