Home --> Expression Systems --> Bacillus licheniformis
You may know that the gram-positive bacterium Bacillus licheniformis has been used for several decades for the industrial production of alpha-amylase and various proteases. It has been classified as GRAS (Generally Recognized As Safe) by the U.S. Food and Drug Administration for the production of alpha-amylase.
An exoprotease deficient B. licheniformis host strain has been specifically tailored for heterologous gene expression. It is asporogenous and gives high extracellular expression levels with minimal loss of product due to proteolytic cleavage subsequent to secretion. To obtain a more genetically stable system after transformation and to increase production levels, the alpha-amylase gene has also been removed.
Vectors for B. licheniformis include both integrating as well as autonomously replicating forms. The essential elements of these vectors are the strong promoter, efficient terminator, suitable signal sequence, and a selection marker. Extensive studies on plasmid stability have shown the vector to be recombinationally stable and segregationally stable during fermentation in the absence of selective pressure.
After supplying the gene to BTR, we will create the construct and optimize its expression. BTR has over 40 years of fermentation development experience, and its capabilities range from shake flasks to pilot-scale. (See our Services, Resources, and Process Development pages for details.) The secretion of desired product into the fermentation medium allows for fairly straightforward downstream processing, eliminating the need for cell disruption or chemical processing techniques. This makes recovery relatively efficient and cost effective.
For more details, contact Reinhardt Rosson at: